| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ORTEGA, Tyler | Edgewater |
NT | |
| 2 | GARCIA, Achilles | University (Orlando) |
NT | |
| 3 | HORNELL, Stephen (Mick) | Orangewood Christian |
1:06.99 | Winter Park - Wildcat Open 2024 |
| 4 | FENTON, Joshaun | University (Orlando) |
NT | |
| 5 | RYCKMAN, Elias | Orangewood Christian |
1:04.98 | Mount Dora Christian Developmental Meet #1 |
| 6 | GONZALEZ, Stanley | Orangewood Christian |
1:11.36 | #2 Mount Dora Developmental Meet |
| 7 | WHITE, Tallen | Edgewater |
NT | |
| 8 | MATHIS, Darren | Boone |
NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BETANCES, Jourdan | Mount Dora Christian |
59.27 | Villages Home Meet #3 |
| 2 | NAPOLI, Mikey | Mount Dora Christian |
59.04 | Bears Invitational |
| 3 | RICHARDSON, darius | Orlando Christian Prep |
55.92 | Jones HS - Tiger Open 2024 |
| 4 | MACKEY, Gilbert | Orlando Christian Prep |
53.95 | Jones HS - Tiger Open 2024 |
| 5 | COOPER, Jayden | Orlando Christian Prep |
54.91 | Mount Dora Christian Developmental Meet #1 |
| 6 | CASTRO, Andrew | Boone |
57.43 | Oak Ridge Invitational |
| 7 | MCCOY, William | Boone |
59.10 | Bears Invitational |
| 8 | PIERRE, Oberson | University (Orlando) |
1:00.13 | Lake Mary Opening Classic 2024 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ROACH, Seth | Mount Dora Christian |
53.10 | |
| 2 | SCOTT, Jacob | West Orange |
50.60 | |
| 3 | MORENO, Dylan | West Orange |
50.50 | |
| 4 | FARLEY, Lukas | West Orange |
50.00 | |
| 5 | GARDNER, Jaden | Winter Park |
50.20 | |
| 6 | WASHINGTON, Ayden | Winter Park |
50.60 | |
| 7 | AUGUSTE, Isiah | Edgewater |
51.15 | Jones HS - Tiger Open 2024 |
| 8 | DIAZ, Gabriel | Winter Park |
53.60 |