| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | East Ridge |
A | 10:00.00 | |
| 2 | George Jenkins |
A | 10:04.12 | FHSAA Class 4A Track & Field State Championships |
| 3 | McKeel |
A | 11:00.76 | FHSAA 2A Region 3 |
| 4 | Lake Wales |
A | 11:20.00 | |
| 5 | Plant City |
A | 11:40.00 | |
| 6 | Largo |
A | DNS | 11:41.98 East Lake Early Bird Invitational |
| 7 | Davenport |
A | 11:49.37 | 11:49.37 Polk County Championship 2023 |
| 8 | Hardee |
A | 11:53.68 | 11:53.68 Clewiston Big Lake Challenge 2023 |
| 9 | Frostproof |
A | 12:00.00 | |
| 10 | Haines City |
A | 12:40.90 | 12:40.90 Lake Region - Thunder Relays2023 |
| 11 | Lake Region |
A | NT |