| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Auburndale | A | NT | |
| 3 | Lake Region | A | NT | |
| 4 | Mulberry | A | DNS | |
| 5 | McLaughlin | A | NT | |
| 6 | Bartow | A | NT | |
| 7 | Bell Creek | A | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Frostproof | A | 46.47 | 46.47 Frostproof - Bulldog Invite 2022 |
| 2 | Celebration | A | DNS | 44.86 Bolles Bulldog Classic |
| 3 | Ridge Community | A | DNS | Lake Buena Vista Home Meet |
| 4 | Kathleen | A | 44.22 | 44.22 Lakeland City Meet 2019 |
| 5 | South Lake | A | 44.31 | 44.31 Freedom High Open 2022 |
| 6 | Newsome | A | 44.41 | FHSAA Class 4A & Para Track & Field State Championships |
| 7 | Plant City | A | 45.50 | |
| 8 | Santa Fe Catholic | A | DNS | FHSAA 1A Region 3 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Lakeland | A | 43.57 | 43.57 6th Annual George Jenkins Invitational 2022 |
| 2 | Niceville | A | 43.23 | East Coast Classic |
| 3 | Lake Wales | A | 42.71 | FHSAA 3A Region 2 |
| 4 | Rockledge | A | 41.57 | Florida Relays |
| 5 | Vero Beach | A | 41.64 | FHSAA Class 4A & Para Track & Field State Championships |
| 6 | George Jenkins | A | DNS | |
| 7 | Fort Meade | A | 43.40 | |
| 8 | Victory Christian (LL) | A | 44.10 |