| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | McKeel | A | DNS | |
| 2 | Newsome | A | NT | |
| 3 | Bartow | A | NT | |
| 4 | Santa Fe Catholic | A | DNS | 4:13.58 Lakeland City Meet 2022 |
| 5 | Vero Beach | A | NT | |
| 6 | Lake Region | A | DNS | |
| 7 | Frostproof | A | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Bell Creek | A | 3:52.71 | 3:52.71 Richard Allen Invitational |
| 2 | Plant City | A | 3:45.00 | |
| 3 | Ridge Community | A | 3:37.23 | Poinciana Invitational |
| 4 | South Lake | A | 3:35.06 | 3:35.06 Freedom High Open 2022 |
| 5 | Victory Christian (LL) | A | 3:35.10 | |
| 6 | West Port | A | DNS | |
| 7 | Lakeland Christian | A | 3:48.00 | |
| 8 | Tampa Bay HEAT | A | 3:55.03 | 3:55.03 6th Annual George Jenkins Invitational 2022 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Lake Wales | A | DNS | 3:29.23 6th Annual George Jenkins Invitational 2022 |
| 2 | George Jenkins | A | 3:29.00 | |
| 3 | Auburndale | A | DNS | FHSAA 3A Region 2 |
| 4 | Niceville | A | 3:20.09 | FHSAA Class 4A & Para Track & Field State Championships |
| 5 | Jones | A | 3:23.64 | FHSAA 3A Region 2 |
| 6 | Celebration | A | 3:24.99 | FHSAA Class 4A & Para Track & Field State Championships |
| 7 | Rockledge | A | 3:29.00 | |
| 8 | Lakeland | A | 3:32.38 | FHSAA 3A Region 2 |