| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Holy Trinity |
A | Roche | Nazareno | Darlington | Mandese | 8:27.92 | Holy Trinity Invitational |
| 2 | Edgewood |
A | Pope | Dehuff | Goyco-Shannon | Sriramineni | 8:47.01 | Rockledge Raider Classic |
| 3 | Astronaut |
A | Boyd | Schram | Potter | Freeman | DNS | Holy Trinity Invitational |
| 4 | Titusville |
A | Allen | Love | Parsons | Van Duyne | 9:37.39 | Astronaut 6 Team Developmental Meet 2025 |