| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 507 | CHEONG, Brayden | SR | Buchholz |
1:05.85 | 2024 River City Relays Classic (Sanctioned Meet) |
| 2 | 149 | WILSON, Antoneo | SR | Eastside |
58.93 | Wildwood Wildcat Spring Break Invitational |
| 3 | 99 | ROCHELLE, Tayeshawn | JR | Gainesville |
55.26 | FSU Relays (High Schools) |
| 4 | 195 | MARTIN, Kristian | SO | Eastside |
58.85 | Wildwood Wildcat Spring Break Invitational |
| 5 | 76 | HARVEY, Dominick | FR | Gainesville |
1:02.23 | 2024 SFHS RAIDERS INVITATIONAL |
| 6 | 220 | HAMILTON, Kewaan | SR | Eastside |
1:07.33 | Columbia High School Jungle Open Meet |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 343 | GROW, Daniel | JR | Oak Hall |
NT | NT |
| 2 | 192 | SMITH, Miles | SO | Eastside |
1:14.30 | Columbia High School Jungle Open Meet |
| 3 | 139 | BADIE, Jozef | SO | Gainesville |
1:07.99 | Columbia High School Jungle Open Meet |
| 4 | 397 | SZABO, Samson | SO | Buchholz |
1:13.77 | 20th Annual - East Coast Classic (FHSAA Sanctioned Meet) |
| 5 | 469 | OUKO-OMONDI, Daniel | FR | Buchholz |
NT | 58.00 |
| 6 | 485 | SMITH, Nigel | JR | Buchholz |
NT | NT |