| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | EDWARDS, Tyjah | JR | Gainesville | 54.33 | Pedro Menendez Falcon Alumni Invite | 
| 2 | KANE, Ryland | SR | Oak Hall | 53.15 | Oak Hall Mini Meet #2 | 
| 3 | GREENE, Jayvian | SO | Oak Hall | 50.07 | Florida Relays | 
| 4 | HILL, Dyoni | SR | Gainesville | 50.93 | Raider Invitational | 
| 5 | PITTS, Payton | JR | Santa Fe | 53.34 | SFHS Mini Meet #2 | 
| 6 | MILLER, Jarrod | SO | Eastside | 54.42 | Raider Invitational | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | EDWARDS, Malik | JR | Gainesville | 55.77 | Raider Invitational | 
| 2 | JOHNSON, Maurice | JR | Eastside | 55.55 | SFHS Mini Meet #2 | 
| 3 | LIVINGSTON, Roderick | FR | Buchholz | 54.77 | Raider Invitational | 
| 4 | BROCKINGTON, Monte | SR | Gainesville | 55.51 | Raider Open | 
| 5 | SKAPINSKI, Mikhail | JR | Buchholz | 55.56 | Raider Invitational | 
| 6 | WILLIAMS, Irvin | SR | Buchholz | 56.14 | Oak Hall Mini Meet #3 | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | GUYDEN, Braylon | SO | Santa Fe | 58.47 | SFHS Mini Meet #2 | 
| 2 | LEWIS, Thomas Leif | SO | P. K. Yonge | 58.02 | SFHS Mini Meet #2 | 
| 3 | MCCOY, Blake | SO | Santa Fe | 57.26 | Raider Invitational | 
| 4 | JOHNSON, Jabere | SO | Eastside | 57.29 | SFHS Mini Meet #2 | 
| 5 | DOBRIN, Shaia | SO | Oak Hall | 58.45 | Oak Hall Mini Meet #3 | 
| 6 | HUNTER, Eli | FR | Newberry | 59.15 | SFHS Mini Meet #2 | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | PATEL, Aakash | FR | St. Francis | NT | NT | 
| 2 | PERRIN, Patrick | FR | St. Francis | 1:17.53 | The Villages Home Meet Two | 
| 3 | PARSONS, Nicolas | SO | Newberry | 1:01.75 | SFHS Mini Meet #2 | 
| 4 | JACKSON, Charles | JR | Newberry | 1:07.44 | SFHS Mini Meet #2 | 
| 5 | INGRAM, Cornelius | FR | Hawthorne | NT | NT | 
| 6 | MILLER, Ma'chir | SR | Buchholz | NT | NT | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | HASKO, Jackson | SO | St. Francis | NT | NT | 
| 2 | BAKER, Terance | FR | Santa Fe | NT | NT | 
| 3 | HILLIER, Harrison | FR | St. Francis | NT | NT | 
| 4 | DOUALEHI, Zavier | SO | P. K. Yonge | NT | NT | 
| 5 | WALDON, Kelby | JR | Eastside | NT | NT | 
| 6 | RUTH, Jailen | JR | Hawthorne | NT | NT | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 4 | THOBY, Jerry | SO | P. K. Yonge | NT | NT |