| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | GIBSON, Kamysia | FR | Gainesville | 51.13 | Don Hall Invitational | 
| 2 | PRESLEY, Nymera | JR | P. K. Yonge | 48.84 | Bob Hayes Classic | 
| 3 | REED, Zuriel | JR | P. K. Yonge | 43.22 | Bob Hayes Classic | 
| 4 | OUKO-OMONDI, Danielle | FR | Gainesville | 47.86 | Don Hall Invitational | 
| 5 | WILLIAMS, Yasmyne | SR | Eastside | 50.59 | Raider Invitational | 
| 6 | WYNN, Savannah | JR | St. Francis | 52.71 | Oak Hall Mini Meet #3 | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | HILLIER, Annabel | SR | St. Francis | 59.63 | Oak Hall Mini Meet #3 | 
| 2 | LIVINGSTON, Arielle | SO | Eastside | 57.50 | Raider Invitational | 
| 3 | CATON, Meagan | SR | St. Francis | 53.96 | The Villages Home Meet Two | 
| 4 | ENOS, Mary | SO | Santa Fe | 56.63 | Raider Fr/So Invitational | 
| 5 | VALDEZ, Kijonea | SR | Eastside | 57.89 | Raider Invitational | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 2 | COLLINS, Jenajah | SR | Buchholz | NT | NT | 
| 3 | EVANS, Janiya | JR | Gainesville | 1:02.39 | Don Hall Invitational | 
| 4 | WILLIAMS, Aniya | FR | P. K. Yonge | NT | NT |