| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | East Lee County |
A | Brooks | Tobler | Graham | Oliver | DNS | |
| 4 | Canterbury (FM) |
A | Jessogne | Heinrichs | Verma | Heinrichs | DNS | |
| 5 | South Fort Myers |
A | Davis | Latus | bernadel | Marcelin | DNS |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mariner |
A | Igess | Mercado | Santiago | Jones | 3:53.41 | Guy Thomas Memorial Classic (FHSAA & NFHS Sanctioned) |
| 2 | Cypress Lake |
A | Ochan | santana | Menezes | Samedi | 3:34.87 | Charlotte Invitational |
| 3 | Evangelical Christian |
A | Morris | Ragsdale | Palma | Wickman | 3:32.76 | FHSAA 1A District 12 |
| 4 | Donahue Academy |
A | Hart | Taddei | Scheck | Freeze | 3:50.76 | 3:50.76 Late Season Dual Meet NHS %26 Gulf Coast |
| 5 | Gulf Coast |
A | Walker | Montanye | Ruggiero | Sagesse | 3:30.65 | Guy Thomas Memorial Classic (FHSAA & NFHS Sanctioned) |
| 6 | Gateway (Fort Myers) |
A | Gray | Barnes | Fernander | Louis | 3:33.83 | FHSAA 2A Region 3 |
| 7 | Estero |
A | Sedmak | Isme | Powell | Rojas | DNS | 2024 South Fort Myers Wolfpack Invitational Sanctioned |
| 8 | Ida Baker |
A | Olmstead | Bayley | Roman | Rivera | 3:38.71 |