| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | BARROSO, Bianca | Victory Christian (LL) |
27.74 | Lakeland City Championships @ George Jenkins 2025 |
| 4 | ASH, Dai'onna | Lake Gibson |
27.67 | Lakeland City Championships @ George Jenkins 2025 |
| 5 | WALKER, Justice | Haines City |
27.60 | Raven Invitational 2025 (Sanctioned) |
| 6 | KRATKY, Sydney | Victory Christian (LL) |
27.63 | Lakeland City Championships @ George Jenkins 2025 |
| 7 | GREEN, E'Mirra | Discovery |
27.67 | 2025 Titan Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | GIBSON, De'Syre | George Jenkins |
27.59 | Lakeland City Championships @ George Jenkins 2025 |
| 3 | WHITE, Caleigha | Lake Wales |
27.52 | Bears Invitational (Regional Qualifier) |
| 4 | KELLY, Imani | Auburndale |
27.45 | Mike Williams Classic @ Lake Region 2025 |
| 5 | LOVELADY, Abigail | McKeel |
27.48 | Lake Region Sprint Hurdle Night 2025 |
| 6 | DAVIS, Jayda | McKeel |
27.55 | Lakeland City Championships @ George Jenkins 2025 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | PAGE, McKenzie | Davenport |
27.19 | Bob English Invitational 2025 |
| 2 | DENSON, Tristan | George Jenkins |
27.15 | Lake Region Sprint Hurdle Night 2025 |
| 3 | HAWKINS, Alahna | All Saints' |
26.94 | Harmony Invitational |
| 4 | JASPER, Aniya | Haines City |
26.46 | Kowboy Invitational |
| 5 | FORSETT, Keiera | Auburndale |
26.91 | 2025 Hawks on the Hill Invitational (Regional Qualifier) |
| 6 | MCGRIFF, Kasondra | McLaughlin | 27.01 | Lake Region Sprint Hurdle Night 2025 |
| 7 | LEMMOND, Addie | Victory Christian (LL) |
27.17 | Lakeland City Championships @ George Jenkins 2025 |
| 8 | HOLLOWAY, Mar'kaila | Lake Gibson |
27.24 | Blue Devil Invitational (Regio |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | STREET, Geriah | George Jenkins |
26.28 | Kowboy Invitational |
| 2 | PAYOUTE, Wizly | Auburndale |
26.15 | Winter Haven Pre-season |
| 3 | CONOLY, Ashari | Winter Haven |
25.59 | Kowboy Invitational |
| 4 | MURRAY, Desirae | Winter Haven |
24.76 | Blue Devil Invitational (Regio |
| 5 | GALLISHAW, Shaia | Winter Haven |
25.56 | Blue Devil Invitational (Regio |
| 6 | ST. FLEUR, Nayelie | Lake Wales |
25.80 | Berkeley Track and Field Invitational |
| 7 | MANAGO, Micaela | Lakeland |
26.23 | Blue Devil Invitational (Regio |
| 8 | LEVERSTON, Leilani | Winter Haven |
26.39 | 2025 Newsome Wolves Invitational |