| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | MONESTINE, Khemani | Davenport |
19.88 | Kowboy Invitational |
| 4 | BUCKLE, Jada | Discovery |
19.62 | Blue Devil Invitational (Regio |
| 5 | TAYLOR, Malaysia | Auburndale |
19.85 | Mike Williams Classic @ Lake Region 2025 |
| 6 | BRANGERS, Kyire | Victory Christian (LL) |
19.92 | Shorecrest Senior Night |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | HAYNES, Temia | Lake Region |
19.54 | Lake Region Sprint Hurdle Night 2025 |
| 3 | LEWIS, Kaitlyn | Auburndale |
18.58 | Lake Region Sprint Hurdle Night 2025 |
| 4 | VASQUEZ, Yalimar | Frostproof |
16.73 | Lake Region Sprint Hurdle Night 2025 |
| 5 | KELLY, Imani | Auburndale |
17.78 | Mike Williams Classic @ Lake Region 2025 |
| 6 | JONES, Naamiah | Lakeland |
18.71 | Bob English Invitational 2025 |
| 7 | FISCHER, Elizabeth | All Saints' |
19.56 | Raven Invitational 2025 (Sanctioned) |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | PAYOUTE, Wiseberly | Auburndale |
16.06 | Mike Williams Classic @ Lake Region 2025 |
| 2 | TOURAY, Sainabou | George Jenkins |
15.46 | Kowboy Invitational |
| 3 | GIBSON, Tori | George Jenkins |
15.04 | Lakeland City Championships @ George Jenkins 2025 |
| 4 | WILLIAMS, Chelsi | George Jenkins |
13.41 | Kowboy Invitational |
| 5 | SYBLISS, Synia | George Jenkins |
14.93 | Lakeland City Championships @ George Jenkins 2025 |
| 6 | WALKER, Mikaila | Lake Wales |
15.42 | Bears Invitational (Regional Qualifier) |
| 7 | WEAVER, Kayla | Winter Haven |
15.89 | Kowboy Invitational |
| 8 | CHOQUETTE, Amber | Victory Christian (LL) |
16.57 | Shorecrest Charger Invitational |