| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | GIDARISINGH, Antwon | McLaughlin | 54.46 | Mike Williams Classic @ Lake Region 2025 |
| 4 | DESIR, Jonny | Haines City |
54.41 | Mike Williams Classic @ Lake Region 2025 |
| 5 | VASQUEZ, Elijah | Ridgeview Global Studies |
54.25 | 2025 Hawks on the Hill Invitational (Regional Qualifier) |
| 6 | ROBINSON, Jayelon | Bartow |
54.35 | ROYAL INVITATIONAL @ The First Academy 2025 |
| 7 | HANSON, Ivan | Ridgeview Global Studies |
54.42 | Bob English Invitational 2025 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | SANDERS, Cameron | George Jenkins |
54.07 | Lakeland City Championships @ George Jenkins 2025 |
| 2 | NICHOLS, Kyron | Mulberry |
53.80 | 2025 Titan Invitational |
| 3 | KELLY, Rashard | Kathleen |
53.09 | Lakeland City Championships @ George Jenkins 2025 |
| 4 | DOUGLAS, Elijah | Tenoroc |
53.05 | Mike Williams Classic @ Lake Region 2025 |
| 5 | ADAMS, Waldemore | Tenoroc |
53.05 | Lakeland City Championships @ George Jenkins 2025 |
| 6 | HILERY, Marshawn | McKeel |
53.45 | Berkeley Track and Field Invitational |
| 7 | KIRKLAND, Zi'yon | Auburndale |
53.89 | Lake Region HS Distance Carnival 2025 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | HANDOFRD, Julius | Winter Haven |
52.42 | 2025 Polk County Freshman Sophomore Championships |
| 2 | DUNCAN, Johan | Auburndale |
52.30 | Lake Region Sprint Hurdle Night 2025 |
| 3 | BATTLE, MARCUS | Lakeland |
52.18 | Tiger Invitational |
| 4 | KEARNEY, Michael | Winter Haven |
51.62 | Mike Williams Classic @ Lake Region 2025 |
| 5 | DANIELS, Ronnie | Lake Wales |
51.71 | Bears Invitational (Regional Qualifier) |
| 6 | BOOKER, Brice | Winter Haven |
52.20 | Mike Williams Classic @ Lake Region 2025 |
| 7 | BRINSON, Dylan | Bartow |
52.34 | Mike Williams Classic @ Lake Region 2025 |
| 8 | FLOWERS, Tristin | George Jenkins |
52.60 | Lakeland City Championships @ George Jenkins 2025 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BORDES, David | Haines City |
50.71 | Kowboy Invitational |
| 2 | MCCALLA, Jah'son | George Jenkins |
50.21 | FSU Relays (High Schools) |
| 3 | JOHNSON, Jayden | Lake Wales |
49.60 | 2025 Titan Invitational |
| 4 | JEAN, Myrone | Lake Wales |
49.18 | Bears Invitational (Regional Qualifier) |
| 5 | GLOVER, Dathan | Santa Fe Catholic |
49.57 | Lakeland City Championships @ George Jenkins 2025 |
| 6 | PARKER, Sha'Ki | George Jenkins |
50.16 | Berkeley Track and Field Invitational |
| 7 | WILLCUT, Peyton | Victory Christian (LL) |
50.28 | Lakeland City Championships @ George Jenkins 2025 |
| 8 | THOMAS, Armani | Tenoroc |
50.85 | Frostproof - The Ridge Open 2025 |