| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Bayside | A | Flores | Placeres | McPherson | Evans | 4:57.03 |
| 4 | Space Coast | A | Jacavage | Mitzlaff | Burton | Webster | 4:51.98 |
| 5 | Titusville | A | Jaszczak | Jaszczak | Mohler | Ebaugh-Santos | 4:55.22 |
| 6 | Eau Gallie | A | Jost | Allen | Salter | Ward | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Cocoa Beach | A | Walker | Shunnarah | Fish | Norman | 4:50.50 |
| 2 | Satellite | A | Hibbard | Markle | Kruv | Coyle | 4:26.14 |
| 3 | Cocoa | A | Tyson | Thomas | Harp | Williams | 4:23.67 |
| 4 | Rockledge | A | Glasco | Buckmaster | Joyce | Dawn | 4:07.55 |
| 5 | Viera | A | Flagg | Jernigan | Ramirez | Thomas | 4:19.34 |
| 6 | Heritage | A | Francois | Pitt | Miller | Baxter | 4:26.08 |
| 7 | Merritt Island | A | DeLena | Lusk | Gibney | Glass | 4:47.45 |
| 8 | Astronaut | A | Sakuma | Treder | Collyer | Hawk | 4:50.68 |