| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Eau Gallie | A | Craig | Watson | Meredith | Mitchell | DNS |
| 3 | Space Coast | A | Chicklinski | Wiler | Miller | Smith | 3:53.79 |
| 4 | Melbourne | A | Bowden | Barnhill | Waters IV | Howell | 3:46.43 |
| 5 | Titusville | A | Hargrove | Jones | Olivo | Etienne | 3:50.33 |
| 6 | Viera | A | Arevelos | Foreman | Carroll | Adams | DNS |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Palm Bay | A | Blackmon | Grady | McCloud | Prude | 3:42.39 |
| 2 | Astronaut | A | Austin | Causey | DeVoss | Lincoln | 3:37.80 |
| 3 | Cocoa | A | Smith | Edgecombe | Holmes | Giles | 3:32.19 |
| 4 | Rockledge | A | Housey | Housey | Buckmaster | Waters | 3:27.85 |
| 5 | Satellite | A | Burns | Wester | Colby | Law | 3:30.03 |
| 6 | Heritage | A | Muncey | Joseph | Redhead | Higgins | 3:34.01 |
| 7 | Merritt Island | A | Hartigan | Dembowski | Shields | Tortolo | 3:40.49 |
| 8 | Bayside | A | Parkes | Derisme | Ferrell | Durant | 3:46.07 |