| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Eau Gallie | A | Ward | Morgan | Lherisse | Jost | NT |
| 4 | Cocoa Beach | A | Rivas | Wolverton | Infante | Fish | 56.68 |
| 5 | Satellite | A | Cohen-Pinsky | Cohen-Pinsky | Rozycki | Negron | 58.33 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Edgewood | A | Laudat | Mueller | Dessauer | Chamberlin | 55.74 |
| 2 | Bayside | A | McPherson | Evans | Garcia | Placeres | 54.54 |
| 3 | Melbourne | A | Rosa | Parsons | Yon | Harms | 51.36 |
| 4 | Rockledge | A | Glasco | King | Devoe | Williams-Goins | 48.70 |
| 5 | Cocoa | A | Parham | Tyson | Harp | Thomas | 49.72 |
| 6 | Heritage | A | Brown | Muncey | Cherichel | Baxter | 52.44 |
| 7 | West Shore | A | Koehler | Weaver | Nemes | Shelton | 55.13 |
| 8 | Merritt Island | A | Lusk | DeLena | Gibney | Glass | 56.62 |