| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Pine Ridge |
B | Jackson | Williams | Hipsher | Weston | 44.22 | Bishop Moore - Carl Friedland 2026 |
| 3 | Atlantic (PO) |
B | Dimeo | ealey | Franck | baciu | 44.99 | SCHS Developmental Meet 1 February 18 2026 |
| 4 | Seabreeze |
B | Fazio | Lomoriello | Kagan | Agee | 44.87 | 12th Annual Craig Speziale Memorial Invitational |
| 5 | Pine Ridge |
D | Benjamin | Burke | Edward | Walker | 44.22 | Bishop Moore - Carl Friedland 2026 |
| 6 | Pine Ridge |
C | Evans | Varmadore | Gordon | Benjamin | 44.22 | Bishop Moore - Carl Friedland 2026 |
| 7 | Deltona |
B | Douglas | Montgomery | Joseph | Deon | 43.94 | Deltona Wolves Kickoff 2026 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Atlantic (PO) |
A | Shaw | Ramos | Harris | Trinidad | 44.99 | SCHS Developmental Meet 1 February 18 2026 |
| 4 | Seabreeze |
A | Wilson | O'Brien | Lugo | McBroom | 44.87 | 12th Annual Craig Speziale Memorial Invitational |
| 5 | Deltona |
A | Lewis | Newton | Baldwin | Morris | 43.94 | Deltona Wolves Kickoff 2026 |
| 6 | Pine Ridge |
A | Blair | Boddie | Braggs | Reed | 44.22 | Bishop Moore - Carl Friedland 2026 |