| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | South Lake |
A | Florence | Espada-Lebron | Lauradin | Neptune | 10:25.07 | FHSAA 4A District 4 |
| 2 | Ocoee |
A | Wynn | Cook | Joseph | Hodges | 10:26.27 | FHSAA 4A District 4 |
| 3 | Boone |
A | Simonds | Varela | Seinfeld | Foley | 10:50.00 | |
| 4 | Jones |
A | Covington | Hall | Barner | Webb | 11:25.89 | FHSAA 3A Region 2 |