| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Edgewater |
A | Robinson-Williams | Jackson | Hutchinson | Barkett | 50.61 | 2026 Dr. Baptiste Invitational hosted by West Orange High |
| 4 | Jones |
A | Sumpter | Forine | Brown | Jones-Graham | 48.24 | FHSAA 3A Region 2 |
| 5 | South Lake |
A | Baldwin | Clements | Grilli | Wong | 49.69 | Evans Development Invitational |
| 6 | Boone |
A | Hardy | Mathis | Terrel | Williams | 52.83 | Wolverine Relays |