| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Boone |
A | Johnson | Parga | Pringle | Wooten | 52.83 | Wolverine Relays |
| 4 | Jones |
A | Webb | Barnett | Rodgers | Hylick | 48.24 | FHSAA 3A Region 2 |
| 5 | Edgewater |
A | Jackson | Fenelon | Robinson-Williams | Haynes | 50.61 | 2026 Dr. Baptiste Invitational hosted by West Orange High |
| 6 | South Lake |
A | Daley | Lambert | Rosario | Bedingfield | 56.00 |