| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 4 | GE, Shanlin | SO | Florida Prep |
22.01 | 2026 Titusville Meet |
| 5 | ONI, Lilah | JR | Orlando_Science_High_School | 20.94 | Royal Invitational @ The First Academy 2026 |
| 6 | JONES, Indra | JR | Florida Prep |
21.98 | 2026 Titusville Meet |
| 7 | DAY, My-Dannie'Ka | JR | Lakeland |
NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | FREEMAN, Cambria | SO | Titusville |
20.71 | 2026 Merritt Island Relays |
| 4 | MCKINSTRY, Alannah | JR | Titusville |
19.54 | Astronaut Invitational 2026 |
| 5 | JEAN-LOUIS, Arianna | JR | Heritage |
17.79 | 2026 Heritage Invitational 2 |
| 6 | ANDERSON, Jolie | JR | Heritage |
18.58 | Tiger Trail Invitational |
| 7 | AYALA, Maliyah | JR | Heritage |
20.17 | 2026 Bayside Invitational |
| 8 | WHITE, Amelia | SO | Melbourne Central Catholic |
20.88 | Holy Trinity Invitational 2026 |