| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | Orlando_Science_High_School | A | Lejuste | Cennet | McDuffie | Prashard | DNS | Royal Invitational @ The First Academy 2026 |
| 5 | Fort Pierce Westwood |
A | Ford | Greer | Lynch | Reynolds | DNS | Vero Beach HS Relay Championships (FHSAA Sactioned) |
| 6 | Master's Academy (Oviedo) |
A | Elkins | Fennell | Silver | Waters | DNS | Lake Mary Invitational 2026 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Melbourne |
A | Alexander | Devitt | Schauman | Kafer | 3:45.83 | FHSAA 4A District 7 |
| 3 | Innovation_Orlando | A | Musovic | Figuero | Martins | Kest | 3:38.84 | Cypress Creek Pre-Season Meet |
| 4 | Cocoa |
A | Orlando | Mcoy | Folston | Robinson | DNS | Tiger Invitational 2026 |
| 5 | Heritage |
A | Parkes | Berrios | Gambrell | Greer | 3:25.89 | FHSAA 4A Region 2 |
| 6 | Titusville |
A | Mckelvin | Williams | Fletcher | Allen | 3:28.62 | FHSAA 2A Region 2 |
| 7 | Lakeland |
A | Campbell | Harris | Jean-Louis | Breckenridge | DNS | FHSAA 4A Region 2 |
| 8 | Melbourne Central Catholic |
A | Smith | Brown | Nail | Crockett | DNS | 2025 Melbourne Central Catholic Invitational |