| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | FORNESS, Parker | FR | Orangewood Christian | NT | |
| 3 | HERNANDEZ, Joshua | 8 | Geneva School | NT | |
| 4 | PORTUGUEZ, Anthony | SO | Bishop Moore | 32.00 | |
| 5 | LOISEAU, Karl | FR | Trinity Prep | 27.52 | Citrus League Championships 2023 |
| 6 | GOOLSBY, Kayleb | SO | Warner Christian | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | THOMPSON, John | SO | Circle Christian | 26.90 | Lake Highland - Highlander Classic 2023 |
| 2 | BRADLEY, Daniel | SO | Windermere Prep | 25.79 | Bishop Moore - Carl Friedland Memorial Track Invite 2023 |
| 3 | RIVERA, Enoch | SO | Circle Christian | 24.84 | 24.84 Bolles Bulldog Classic |
| 4 | JAMES, Khamari | JR | Windermere Prep | 24.76 | |
| 5 | VALY, Nathan | FR | Oasis Christian Academy | 24.82 | Lake Region Sprint Hurdle Night 2023 |
| 6 | BASS, Luca | SR | Orangewood Christian | 24.89 | Bishop Moore - Larry Kelly Invitational 2023 |
| 7 | OLEYAR, Cory | SO | Windermere Prep | 26.17 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BANCALARI, Augustin | SR | Circle Christian | 24.71 | Falcon Friday Night 13 |
| 2 | HATCHETTE, Mabon | SO | Trinity Prep | 24.12 | Panther Invitational |
| 3 | VAN DYKE, Oliver | SR | Winter Park | 23.80 | |
| 4 | JUSME, William | SO | Bishop Moore | 23.57 | Winter Park - Brian Jaeger Elite Classic 2023 |
| 5 | CARTER, CJ | JR | Deptford | 23.70 | |
| 6 | WHITE, Charles | SR | Geneva School | 23.81 | Winter Park - Brian Jaeger Elite Classic 2023 |
| 7 | FORD, Elijah | SR | Warner Christian | 24.25 | George Janan SC Invitational M |
| 8 | BULGER, Elijah | JR | Orangewood Christian | 24.74 | Mount Dora Christian Invitational Meet #2 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GARDNER, Jaden | SO | Winter Park | 23.30 | Winter Park - Brian Jaeger Elite Classic 2023 |
| 2 | HENDRIX, Campbell | JR | Geneva School | 23.07 | Mount Dora Christian Invitational |
| 3 | WAYMAN, Darryl | JR | Deptford | 22.70 | |
| 4 | TAYLOR, Janius | JR | Victory Christian (LL) | 22.27 | Shorecrest Senior Night |
| 5 | MCCLENDON, Marlon | SR | Victory Christian (LL) | 22.29 | FHSAA 1A Region 3 |
| 6 | BERRY, Christian | JR | Deptford | 22.90 | |
| 7 | ORR, Rashad | JR | Victory Christian (LL) | 23.12 | George Jenkins - Bob English Invitational 2023 |
| 8 | WASHINGTON, Ayden | JR | Winter Park | 23.47 | Tohopekliga Tigers Invitational |