| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Angeline Academy | A | 4:01.79 | Freedom Spring Break Invitational |
| 4 | George Steinbrenner |
A | 3:52.78 | Tampa Jesuit Meet 4 |
| 5 | Calvary Christian (Clr) |
A | 3:55.62 | East Lake Early Bird Invitational (Approved Qualifier) |
| 6 | Mitchell |
A | 4:07.76 | Anclote High School Invite |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Cypress Creek (Wesley Chapel) |
A | 3:47.00 | Freedom Spring Break Invitational |
| 2 | Sunlake |
A | 3:43.85 | West Coast Invite |
| 3 | Alonso |
A | 3:41.95 | East Lake Early Bird Invitational (Approved Qualifier) |
| 4 | Wiregrass Ranch |
A | 3:37.01 | Coyote Invitational |
| 5 | East Lake |
A | 3:37.06 | Newsome Wolves Invitational 2026 (Approved Qualifying Meet) |
| 6 | River Ridge |
A | DNS | 62th Annual Ed Wells, Jr Kiwanis Invitational 2026 |
| 7 | Lecanto |
A | 3:45.46 | South Sumter High School Raider Open |
| 8 | Bishop McLaughlin |
A | 3:50.77 | Pirate Booty Invitational- Approved Qualifier |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Gaither |
A | 3:36.23 | 2026 Nash Higgins Relays |
| 2 | Wharton |
A | 3:33.89 | Newsome Wolves Invitational 2026 (Approved Qualifying Meet) |
| 3 | Hillsborough |
A | 3:25.25 | TFRRS 02/14/26 - 3:40 |
| 4 | Newsome |
A | 3:20.99 | 2026 Manuel Griffin Fraundorfer Relays |
| 5 | Largo |
A | 3:23.48 | Mustang Invitational |
| 6 | Wesley Chapel |
A | 3:26.47 | Freedom Spring Break Invitational |
| 7 | King |
A | 3:34.28 | Newsome Wolves Invitational 2026 (Approved Qualifying Meet) |
| 8 | Robinson |
A | DNS | 2026 Manuel Griffin Fraundorfer Relays |