| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Sarasota |
A | Greenwell | Larson | Wheeler | Freymann | DNS | |
| 2 | Riverview (Sarasota) |
A | Tenaglia | Dynan | Taylor | Culpepper | 11:18.66 | Bobcat Relays |
| 3 | Venice |
A | Heaney | Leitzen | Schmit | Shaffaval | 11:29.81 | Bobcat Relays |
| 4 | North Port |
A | Meza | Rokovets | Stubbings | Velazquez-Rodriguez | 11:35.58 | |
| 5 | Clearwater Central Catholic |
A | Harrington | Remington | Pollitzer | Abernathy | 11:58.34 | Lipham Relays/St. Petersburg%2C FL |
| 6 | Parrish |
A | Minteer | Mitchell | Porter | Goelz | 12:00.87 | Bobcat Relays |
| 7 | Braden River |
A | Staton | Pham | Trombetta | Zink | 12:39.58 | 59th ANNUAL EAGLE INVITATIONAL |
| 8 | Out-of-Door |
A | Randall | Prete | Hillary | Zukiewicz | NT |