| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Braden River |
A | Mondezie | Duprey | Anthou | Edwards | DQ | |
| 3 | Palmetto |
A | Pompey | Williams | Phillips | Gates | 51.42 | 51.42 Palmetto High Invitational |
| 4 | Sarasota |
A | Snelson | Testa | Bragg | Crisp | 54.29 | 54.29 Sarasota Riverview - Ram Invite 2025 |
| 5 | Out-of-Door |
A | Bloodworth | Donaghy | Gindoff | Wyman | DNS |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Venice |
A | Jones | barrett | Daczewitz Leckie | Hunter | 50.90 | |
| 2 | Riverview (Sarasota) |
A | Wilcox | Sanders | Haygood | Sanders | 50.15 | FHSAA 4A Region 3 |
| 3 | Cardinal Mooney |
A | Armstrong | Harrell | Hroncich | Cangro | DQ | 49.58 Larry Richardson Memorial Invitational |
| 4 | Parrish |
A | Bacon | Backes | Williams | Price | 50.00 | |
| 5 | North Port |
A | Sandford-Holmes | Louty | Service | Murray | 50.20 | |
| 6 | Clearwater Central Catholic |
A | Manapin | Murack | Capco | McFeeture | 51.39 | Keswick Crusader Invitational |