| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Cardinal Mooney |
A | Cesar | Kenney | Dau | Hawkins | 3:49.96 | FHSAA 2A District 10 |
| 3 | Braden River |
A | Radcliffe | Sloan | Kien | Zink | 3:46.77 | 3:46.77 North Port Invitational 2025 |
| 4 | Clearwater Central Catholic |
A | Liebel | Rodriguez | Swiderek | Murack | 3:48.66 | FHSAA 2A Region 3 |
| 5 | Out-of-Door |
A | Prete | Bowling | Randall | Hillary | DNS |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Riverview (Sarasota) |
A | Schnaeidr | Dynan | Ramalho | Quamina | 3:35.74 | 3:35.74 Larry Richardson Memorial Invitational |
| 2 | Palmetto |
A | Ashton | Sanders | Starling | Roberson | DNS | 3:34.51 Hurricane Invitational Track and Field Meet |
| 3 | Parrish |
A | Mitchell | Simeus | Andrew | Ceres | 3:21.13 | FHSAA 4A Region 3 |
| 4 | Venice |
A | Miller | Henderson | Lombardi | Evans | 3:32.10 | |
| 5 | Sarasota |
A | Bowen | Russo | Freymann | Wheeler | 3:35.47 | 3:35.47 Bobcat Relays |
| 6 | North Port |
A | Stubbings | Velazquez-Rodriguez | Meza | Rollins | 3:40.00 |