| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Sarasota |
A | Altine | Fazio | Johnson | Richey | 4:26.10 | Bobcat Relays |
| 3 | Cardinal Mooney |
A | Buttle | Lewicki | Lewicki | Xinidis | 4:01.23 | Bobcat Relays |
| 4 | Braden River |
A | Boyd | Pham | Radcliffe | Trombetta | 4:02.79 | Bobcat Relays |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Venice |
A | Miller | Lombardi | Henderson | Young | 3:55.40 | |
| 2 | Riverview (Sarasota) |
A | Achillie | Minnis | Dynan | Blackden | 3:50.39 | Bobcat Relays |
| 3 | Clearwater Central Catholic |
A | Kurzwell | Tillman | Bell | Rodriguez | 1:40.00 | |
| 4 | North Port |
A | Service | Carius | Zdrojowy | Myers | 3:50.00 | |
| 5 | Parrish |
A | Lasquite | Case | Shatkus | Miller | 3:54.88 | Bobcat Relays |