| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Out-of-Door |
A | 4:47.77 | Larry Richardson Memorial Invite |
| 3 | Lemon Bay |
A | 4:33.87 | Tarpon Classic |
| 4 | North Port |
A | 4:40.00 | |
| 5 | Classical Academy (Sarasota) |
A | DNS | Lakewood Ranch Invitational 2026 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Cardinal Mooney |
A | 4:25.24 | FHSAA 2A District 10 |
| 2 | Sarasota |
A | 4:14.13 | FHSAA 4A Region 3 |
| 3 | Riverview (Sarasota) |
A | 4:08.25 | Lakewood Ranch Invitational 2026 |
| 4 | Venice |
A | 4:11.75 | Lakewood Ranch Invitational 2026 |
| 5 | Parrish |
A | 4:20.00 | |
| 6 | Charlotte |
A | 4:32.25 | 4:32.25 Bobcat Relays |