| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | KRATKY, Logan | Victory Christian (LL) |
53.96 |
| 2 | DAVIS , Ryan | Ridge Community |
53.92 |
| 3 | VASQUEZ, Elijah | Ridgeview Global Studies |
53.77 |
| 4 | GRAY, Alex | McKeel |
53.72 |
| 5 | DESIR, Jonny | Haines City |
53.77 |
| 6 | PATINO, Simitrio | Ridge Community |
53.91 |
| 7 | MAISONET, Yariel | Discovery |
53.93 |
| 8 | LOUIS, Tyler | Lake Gibson |
53.96 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | GIDARISINGH, Antwon | McLaughlin | 53.61 |
| 2 | MOORE, Brandon | Winter Haven |
53.53 |
| 3 | SCARLETT, Vernon | McLaughlin | 53.39 |
| 4 | TYLER, Da' Money | Auburndale |
53.32 |
| 5 | MARQUEZ, Antonio | Winter Haven |
53.34 |
| 6 | FURMAN, Jaidon | Tenoroc |
53.47 |
| 7 | YANEZ-DOMINGU, Ismael | Ridge Community |
53.56 |
| 8 | MORALES, Adrian | George Jenkins |
53.63 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | CAMPBELL, DeAndrae | Winter Haven |
52.76 |
| 2 | BECK, D'Anthony | George Jenkins |
52.59 |
| 3 | BOOKER, Brice | Winter Haven |
51.86 |
| 4 | CANNON, Erick | George Jenkins |
51.60 |
| 5 | DANIELS, Ronnie | Lake Wales |
51.71 |
| 6 | ADAMS, Waldemore | Tenoroc |
52.45 |
| 7 | VEGTER, Jayden | McKeel |
52.66 |
| 8 | ROBINSON, Jayelon | Bartow |
52.94 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | TARVER, Jordan | Auburndale |
51.11 |
| 2 | BRINSON, Dylan | Bartow |
51.02 |
| 3 | BORDES, David | Haines City |
50.71 |
| 4 | PARKER, Sha'Ki | George Jenkins |
49.75 |
| 5 | MANNING, JaDarrion | Tenoroc |
50.69 |
| 6 | PUGH, Raeshaun | Lake Wales |
50.95 |
| 7 | WALKER, Marcus | Lake Wales |
51.08 |
| 8 | POOLE, Lamarir | Tenoroc |
51.54 |