| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | NIEVES, Josean | SR | Discovery | NT | |
| 4 | REVUELTAS, Jayden | FR | Lake Region | NT | |
| 5 | MCCRAY, Tory | FR | Haines City | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | CARIOTTE, Chris | SO | Discovery | NT | |
| 3 | KEY-HARRINGTON, Quran | JR | Winter Haven | 1:06.65 | Berkley Track and Field Invitational |
| 4 | HYDE, Dominic | SR | Lakeland | 1:09.11 | Tohopekliga Tigers Invitational |
| 5 | JOSEPH, John | SR | Haines City | 1:12.07 | Oak Ridge Invitational |
| 6 | ALVARADO, Cipriano | SR | Frostproof | 1:16.00 | |
| 7 | MERCADO, Josias | SO | Discovery | NT |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PITTMAN, Daiveon | JR | Frostproof | 1:10.45 | |
| 2 | CONNER, Dominique | FR | Haines City | 1:12.54 | Oak Ridge Invitational |
| 3 | CAMPBELL, Christopher | JR | Ridge Community | 1:06.28 | Early Hawk Invitational |
| 4 | BERRIOS, Pedro | SR | George Jenkins | 1:04.57 | Lakeland City meet @ George Jenkins 2023 |
| 5 | PIERRE-LOUIS, Lensley | JR | Lake Region | 1:06.00 | |
| 6 | MCWORTER, Seth | SR | Davenport | 1:06.42 | 12th Annual Hawks on the Hill Invitational |
| 7 | SKINNER, Omari | SR | George Jenkins | 1:07.88 | Berkley Track and Field Invitational |
| 8 | COBBETT, Ka'Mari | SR | Lakeland | 1:09.21 | Tohopekliga Tigers Invitational |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DYER, Sytrevion | JR | Lakeland | 1:02.89 | Tohopekliga Tigers Invitational |
| 2 | COLE, Kamarion | JR | Auburndale | 1:01.27 | 12th Annual Hawks on the Hill Invitational |
| 3 | ALLEN, Jacob | SR | Lakeland Christian | 59.77 | Mustang Invite |
| 4 | VALENTIN, Joshuwa | SR | George Jenkins | 57.15 | St. Cloud Invitational |
| 5 | PHILPOT, Cooper | JR | Lakeland Christian | 58.56 | Berkley Track and Field Invitational |
| 6 | TULLIS, Marlon | SO | Winter Haven | 1:00.67 | Kowboy Invitational |
| 7 | RODRIGUEZ, Jomar | SR | McKeel | 1:02.61 | Tohopekliga Tigers Invitational |
| 8 | WILLIAMS, Malachi | SR | Lakeland Christian | 1:03.63 |