| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Boone |
A | Alexander | Pullum | Vanos | Sahaydak | 9:45.70 | Bishop Moore - Carl Friedland 2026 |
| 2 | Mount Dora |
A | Bray | Quarnoccio | Patel | Lebron | 10:29.77 | St. Cloud Invitational (Qualifier) |
| 3 | Trinity Prep |
A | Hennebery | Carlin | Gonzalez | Del Vecchio | 10:39.01 | Lake Highland - Highlander Classic 2026 |
| 4 | First Academy (Orlando) |
A | Anderson | Cruz Arroyo | Guss | Morse | 11:06.03 | St. Cloud Invitational (Qualifier) |
| 5 | Lake Highland Prep |
A | Botic | Socarras | Menozzi | Patel | 11:48.00 | |
| 6 | Hagerty |
A | NT |