| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Seminole (Sanford) |
A | 8:08.18 | FHSAA 4A Region 1 |
| 2 | Oviedo |
A | 8:18.63 | FHSAA 4A District 3 |
| 3 | Lake Mary |
A | 8:21.01 | FHSAA 4A Region 1 |
| 4 | Matanzas |
A | 8:39.18 | Valentine Opener |
| 5 | Wekiva |
A | 8:41.84 | FHSAA 4A District 4 |
| 6 | DeLand |
A | DNS | 21st Annual East Coast Classic |
| 7 | Freedom (Orlando) |
A | 8:57.19 | St. Cloud Invitational |
| 8 | Master's Academy (Oviedo) |
A | 9:09.78 | FHSAA 1A Region 2 |
| 9 | Father Lopez |
A | 9:30.75 | FHSAA 1A District 6 |