| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Tavares |
A | Nava Garcia | Noll | Noll | Hartley | 10:27.81 | Bishop Moore - Carl Friedland 2026 |
| 2 | Hagerty |
A | Keefer | Daly | Brewster | David | 10:37.88 | Sanford-Seminole Invitational 2026 |
| 3 | Trinity Prep |
A | Hennebery | Carlin | Tanielian | Del Vecchio | 10:44.78 | Bishop Moore - Carl Friedland 2026 |
| 4 | First Academy (Orlando) |
A | Anderson | Ayla | Guss | Martinez | 11:06.03 | St. Cloud Invitational (Qualifier) |
| 5 | Lake Highland Prep |
A | Botic | Menozzi | Socarras | Chopra | 11:07.26 | FA LIONS INVITATIONAL |
| 6 | Geneva School |
A | Blanton | Shepherd | Mosquera | Puopolo | 12:19.79 | The Geneva School Developmental Meet #3 2026 |