| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Geneva School |
A | Mitchell | Howell | Faith | Demo | DNS | The Geneva School Developmental Meet #3 2026 |
| 2 | Trinity Prep |
A | Sagotsky | Nave | Greenwald | Lou | 3:51.21 | Bishop Moore - Carl Friedland 2026 |
| 3 | Hagerty |
A | Schneider | Labonne | Perry | Thompson | 3:38.88 | Hagerty Track and Field Invitational 2026 |
| 4 | Lake Highland Prep |
A | Henderson | Allen | Dishman | Schier | 3:26.98 | 2026 Dr. Baptiste Invitational hosted by West Orange High |
| 5 | Menendez |
A | Sivyer | Johnson | Jackson | Hedrick | 3:35.65 | 18th Annual George Janan Spruce Creek Invitational |
| 6 | First Academy (Orlando) |
A | Woods | Bonet | Goodwin | Ivsic | DNS | FA LIONS INVITATIONAL |
| 7 | Astronaut |
A | Ebaugh | Robinson | Boring | Porter | 3:51.44 | TFRRS 02/20/26 - 3:46.0 |