| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MCCANTS, Taylor | FR | Bartow | NT | |
| 2 | TUCKER, Jaleisya | FR | Bartow | 1:18.87 | 1:18.87 Polk County Championship 2023 (4/08/2023) |
| 3 | CARDEC, Kathryn | FR | Lake Gibson | 1:14.09 | 1:14.09 Lakeland City meet @ George Jenkins 2023 (3/30/2023) |
| 4 | ACOSTA, Angelique | SO | Celebration | 1:09.63 | 1:09.63 Eye of the Storm Invitational (4/07/2023) |
| 5 | DELVA, Michelle | SO | Poinciana | 1:10.59 | 1:10.59 Coach Mac Monday Madness (3/20/2023) |
| 6 | HASSAN, Donia | SO | George Jenkins | 1:14.78 | 1:14.78 Lakeland City meet @ George Jenkins 2023 (3/30/2023) |
| 7 | CRUZ BAEZ, Rousfrangely | FR | Poinciana | 1:21.55 | 1:21.55 Early Hawk Invitational (2/24/2023) |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | JOHNSON, Eva | SR | Poinciana | 1:07.76 | 1:07.76 Orange Belt Conference Championships (4/12/2023) |
| 2 | HARRISON, Caley | FR | Plant City | 1:06.63 | 1:06.63 Wildcat Invitational (2/25/2023) |
| 3 | ODIKPA, Grace | SO | Bartow | 1:05.97 | 1:05.97 Davenport invitational 2023 (3/25/2023) |
| 4 | MOFFIT, Ayla | SO | Celebration | 1:05.25 | 1:05.25 Orange Belt Conference Championships (4/12/2023) |
| 5 | MEADOWS, Sophia | FR | Celebration | 1:05.59 | 1:05.59 Eye of the Storm Invitational (4/07/2023) |
| 6 | BODDIE, Jaedanyree | SO | Winter Haven | 1:06.01 | 1:06.01 Tohopekliga Tigers Invitational (3/25/2023) |
| 7 | DALTIRUS, Dena | SO | Ridge Community | 1:07.49 | 1:07.49 Davenport invitational 2023 (3/25/2023) |
| 8 | HARRIS, Anecia | FR | Plant City | 1:09.03 | 1:09.03 WC Federal Division (4/05/2023) |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HUTCHINS, Adaisha | FR | Plant City | 1:04.91 | 1:04.91 George Steinbrenner Invitational (4/01/2023) |
| 2 | BRYANT, Ryla | SR | Lake Gibson | 1:01.07 | 1:01.07 Davenport invitational 2023 (3/25/2023) |
| 3 | CANTY, Aliyah | JR | Winter Haven | 58.46 | 58.46 Polk County Championship 2023 (4/08/2023) |
| 4 | HENRY, Dashanique | SO | Winter Haven | 54.52 | 54.52 Polk County Championship 2023 (4/08/2023) |
| 5 | MCBRIDE, Jaa'Ln | JR | George Jenkins | 57.31 | 57.31 FSU Relays (High Schools) (3/24/2023) |
| 6 | STREET, Geriah | FR | George Jenkins | 58.81 | 58.81 Polk County Championship 2023 (4/08/2023) |
| 7 | GLASGOW, Shanique | SR | Ridge Community | 1:02.27 | 1:02.27 Polk County Championship 2023 (4/08/2023) |
| 8 | ASH, Dai'onna | SO | Lake Gibson | 1:05.07 | 1:05.07 Windermere Meet of Champions Classic 2023 (2/25/2023) |