| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | New Smyrna Beach |
A | 10:08.98 | 5-Star Conference |
| 2 | Winter Park |
A | 10:15.48 | 2nd Annual Tammy Lafayette Relays 2026 |
| 3 | NSU University |
A | 10:31.78 | FHSAA 2A Region 4 |
| 4 | West Port |
A | 10:33.49 | 2025 MAC Track and Field Championships |
| 5 | Mainland |
A | 10:37.30 | Charlie Harris Relay |
| 6 | East River |
A | 10:56.20 | FHSAA 4A District 3 |
| 7 | Spruce Creek |
A | 11:00.09 | |
| 8 | Spruce Creek |
B | 12:00.09 |