| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Winter Park |
A | 4:05.41 | FHSAA 4A Region 1 |
| 2 | New Smyrna Beach |
B | 4:03.26 | FHSAA 3A Region 2 |
| 3 | IMG Academy |
A | 4:00.00 | |
| 4 | Wekiva |
A | 3:51.76 | FHSAA 4A Region 1 |
| 5 | West Port |
A | 3:51.88 | Florida Relays |
| 6 | New Smyrna Beach |
A | 4:03.26 | FHSAA 3A Region 2 |
| 7 | Deltona |
A | 4:03.47 | FHSAA State Track & Field Championships (3A) |
| 8 | NSU University |
A | 4:05.97 | FHSAA State Track & Field Championships (2A/Para-Athlete) |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Lake Worth |
A | 4:40.12 | Spanish River HS Invitational Track and Field 2026 |
| 2 | Spruce Creek |
A | 4:35.09 | |
| 3 | East River |
A | 4:14.28 | FHSAA 4A District 3 |
| 4 | Mainland |
A | 4:12.72 | Embry-Riddle Running Elements Classic High School |
| 5 | East River |
B | 4:14.28 | FHSAA 4A District 3 |
| 6 | Menendez |
A | 4:15.00 | Embry-Riddle Running Elements Classic High School |
| 7 | University (OC) |
A | 4:37.11 | Sanford-Seminole Open 2025 |
| 8 | Spruce Creek |
B | 4:50.90 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Menendez |
B | NT | |
| 4 | Seabreeze |
A | 4:57.87 | Deltona Invitational 2025 |
| 5 | Seabreeze |
B | 4:57.87 | Deltona Invitational 2025 |
| 6 | Orlando Christian Prep |
A | NT |