| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Nova Southeastern |
A | Butler | Mannino | Jordan | James | 3:57.06 | Nova Southeastern University Alumni Invitational |
| 2 | Bethune-Cookman |
A | Trim | Middleton | Green | Morillo | 3:55.45 | |
| 3 | Rowan |
A | Jones | Franklin | O'Sullivan | Pinkett | 3:52.00 | |
| 4 | SC State |
A | Williams | Asbury | Singleton | South | 3:45.31 | 2025 MEAC Outdoor Track & Field Championships |
| 5 | A | Stanford | Bryant | Hall | Wooten | DNS | Pre-National | |
| 6 | Embry-Riddle |
A | Best | Roth | Triner | McBride | 3:55.30 | The Legends of Alachua County Invitational College TEAMS |
| 7 | SC State |
B | Miller | Toussaint | Heard | Joe | DNS | |
| 8 | Lynn |
A | Giustino | Bray | Smith | Betts | 3:58.16 | 2025 Embry-Riddle Running Elements Classic College |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Bethune-Cookman |
B | Robinson | Franco | Harper | Simmons | 4:10.24 | |
| 2 | Lynn |
B | Howard | Lombardo | Akan | Brown | 4:10.00 | |
| 3 | Nova Southeastern |
B | Bittner | Davies | Kirk | Burkhart | 4:05.00 | |
| 4 | USC-Beaufort |
A | Edwards | Burnside | Thomas | Olsen | DNS | 2026 Peach Belt Conference Championship |
| 5 | SC State |
C | Smalls | Bundrage | Williams | Williams | DNS | |
| 6 | Oglethorpe |
A | Wright | Wright | Rose | Thomas | 4:05.09 | 2025 Embry-Riddle Running Elements Classic College |
| 7 | Southeastern U. |
A | Brown | Gonzalez | Morley | Coggins | 4:10.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Lynn |
C | Velasquez | Daley | Selke | Hicks | DNS | |
| 4 | Bethune-Cookman |
C | White | Gee | Brown | Bonsu | 4:12.10 | |
| 5 | Rowan |
B | Rizzo | Moore | Walker | Wernik | DNF |