| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Pine Ridge |
A | Levert | Hernandez | Myers | Pomales | 9:41.33 | Bishop Moore - Carl Friedland 2026 |
| 2 | Lake Howell |
A | Spence | Sphabmixay | Batres | Smith | 10:19.34 | Hagerty Track and Field Invitational 2026 |
| 3 | Oviedo |
A | deArmas | Diaz-Quinones | Bardwell | Tamassan | NT | |
| 4 | University (OC) |
A | Kwamina | Cordero | Rivera | Ortiz | DNS |