| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Oviedo |
A | Chisari | Cruz | Maheia | Patino | NT | |
| 3 | Pine Ridge |
A | Coffy | Edwards | Williams | Menefee | 56.33 | Bishop Moore - Carl Friedland 2026 |
| 4 | Lake Howell |
A | Anglin | Jacobus | Knighton | Ramon | 52.62 | Ocoee Relays |
| 5 | Deltona |
A | Andre | Assent | Hill | Stafford | 55.78 | Deltona Wolves Kickoff 2026 |
| 6 | University (OC) |
A | Buchanan | Garcia-Ocasio | Smith | Maleka | 58.40 | SCHS Developmental Meet 1 February 18 2026 |