| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lake Mary |
A | Gihon | Slater | King | Barrett | 10:59.34 | Lake Mary Invitational 2026 |
| 2 | Seminole (Sanford) |
A | Laurenceau | Coffee | Laurenceau | Heilman | 11:26.24 | Sanford-Seminole Invitational 2026 |
| 3 | University (OC) |
A | Grieves | Horrell | Guzman | Bryant | 12:50.75 | SCHS Developmental Meet 1 February 18 2026 |
| 4 | Mainland |
A | NT |