| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | OCPS ACE | A | Jones | Speed-Reynolds | Wright | Hunter | DNS | |
| 3 | College Park Middle School | A | Liable | Sessler | Morrs | Pierce | 4:17.00 | |
| 4 | Maitland MS | A | Lumucso | Paul | Mims | Peck | 4:12.00 | |
| 5 | Glenridge Middle School | A | Hurd | Chudgar | Poole | Buza | 4:16.00 | |
| 6 | Audubon Park School K-8 | A | Bleakley | Park | Drake | McCormack | 4:19.30 | |
| 7 | Union Park Middle School | A | Negron Ortiz | Henry | Robinson | Martinez | DNS |