| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Edgewood | A | Caple | Dehuff | Tran | Woodrich | DNS | |
| 4 | Titusville | A | Bonta | Curry | Dandridge | Jones | 3:46.79 | |
| 5 | Melbourne | A | Kelly | Chang | Stevens | Kempf | 3:53.81 | |
| 6 | West Shore | A | Plechaty | Panchal | Falls | Collins | 4:30.81 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Merritt Island | A | Verpaele | Geesey | Moss | DeReese | 3:38.14 | |
| 2 | Heritage | A | Silfin | Stanley | Moerch | Muncey | 3:34.82 | |
| 3 | Bayside | A | Reynolds | Craig | Parkes | Reynolds | 3:30.54 | |
| 4 | Rockledge | A | Bell Jr | Carter | Alexander | Carter | 3:24.72 | |
| 5 | Cocoa | A | Edgecombe | Hawkins | Dobbs | Holmes | 3:27.89 | |
| 6 | Viera | A | Nelson | Morrell | Meyer | Handal | 3:30.79 | |
| 7 | Astronaut | A | Tilford | Lincoln | Peters | Beachem | 3:35.57 | |
| 8 | Satellite | A | Larrick | Mahorner | Butler | Carr | 3:40.94 |