| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Edgewood | A | Diaz | Elston | Cabrera | Esemuede | 48.18 | |
| 3 | Astronaut | A | Avelar | Beachem | Peters | Lincoln | 45.30 | |
| 4 | Merritt Island | A | Geesey | Verpaele | Moss | DeReese | 44.50 | |
| 5 | Titusville | A | Christian | Martin | Green | Smith | 44.91 | |
| 6 | Heritage | A | Dorcin | Arlin | Harrison | Warren | 45.91 | |
| 7 | West Shore | A | Li | Johnson | Richardson | Matias | DNF |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Viera | A | McPherson | Meyer | Mills | Gentile | DNS | |
| 2 | Palm Bay | A | Johnson | DiBenedetto | Pittman | Official | 43.63 | |
| 3 | Eau Gallie | A | Gonzalez Washington | Blackmon | Miller | Barbaro | 43.15 | |
| 4 | Rockledge | A | Green | Carter | Combs III | Heyward | 42.54 | |
| 5 | Cocoa | A | Edgecombe | Holmes | Hawkins | Dobbs | 42.58 | |
| 6 | Bayside | A | Josephs | Craig | Parkes | Reynolds | 43.28 | |
| 7 | Melbourne | A | Howell | Brown | Snider | Barnhill | 44.08 | |
| 8 | Space Coast | A | Espinosa | Smith | Rainey | Ayliffe | 44.33 |