| Lane | Team | Squad | Seed |
|---|---|---|---|
| 1 | Boone | A | 8:15.49 |
| 2 | Tohopekaliga | A | 8:29.49 |
| 3 | Oviedo | A | 8:37.84 |
| 4 | Oak Ridge | A | 8:38.41 |
| 5 | Bishop Moore | A | 8:40.86 |
| 6 | Cypress Creek | A | 8:50.00 |
| 7 | Colonial | A | 8:51.15 |
| 8 | Winter Park | A | 8:55.50 |
| 9 | South Lake | A | 8:59.24 |
| 10 | Eustis | A | 9:05.71 |
| 11 | Freedom (Orlando) | A | 9:06.77 |
| 12 | Horizon | A | 9:16.13 |
| 13 | Timber Creek | A | 10:22.00 |
| 14 | First Academy (Orlando) | A | NT |
| 15 | Geneva School | A | NT |
| 16 | Lake Mary | A | NT |