| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 3 | GRANTHAM, Emily | Boone | NT |
| 4 | BAMFO, Rhianna | Freedom (Orlando) | 32.69 |
| 5 | BLANTON, Kate | Geneva School | 31.77 |
| 6 | DA SILVA, Julia | First Academy (Orlando) | 32.32 |
| 7 | COMBS, Callie | Tavares | 32.92 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | DAVIS, Aliyah | Freedom (Orlando) | 31.70 |
| 2 | DAVIS, Nadia | Freedom (Orlando) | 31.45 |
| 3 | RIVERA, Jailynn | Tohopekaliga | 30.72 |
| 4 | INCINELLI, Ella | Geneva School | 30.32 |
| 5 | GONZALES, Layla | South Lake | 29.55 |
| 6 | SANDIFER, Aubrie | Oviedo | 30.00 |
| 7 | SUKUT, Catherine | Colonial | 30.67 |
| 8 | MALDONADO, Luna | Eustis | 31.27 |
| 9 | LOZADA DA SILVA, Anastasia | First Academy (Orlando) | 31.67 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | CHAMBERS, Jayla | Boone | 29.22 |
| 2 | CUEVAS, Emma | Timber Creek | 28.93 |
| 3 | BARRERO, Annie | Boone | 28.79 |
| 4 | SMITH, Alyssa | University (Orlando) | 28.63 |
| 5 | GOTAY, Julianna | Winter Park | 28.26 |
| 6 | YOERGER, Alyssa | Bishop Moore | 28.49 |
| 7 | MATTHEWS, Starlet | Tohopekaliga | 28.71 |
| 8 | WAHL, Aliyah | Oviedo | 28.83 |
| 9 | DIAZ MONGE, Kirsy | Tohopekaliga | 29.05 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | WAHL, Alyssa | Oviedo | 28.24 |
| 2 | PHILLIPS, Shila | Winter Park | 28.06 |
| 3 | NEWHARTZ, Scarlett | Geneva School | 28.05 |
| 4 | WALTERS, Gabrielle | First Academy (Orlando) | 27.95 |
| 5 | BROOKS, Monica | Lake Mary | 27.81 |
| 6 | KAHN, Carly | Lake Highland Prep | 27.83 |
| 7 | ANDERSON, Mia | Horizon | 28.00 |
| 8 | RUFFINS, Lauren | Tavares | 28.06 |
| 9 | EDWARDS, Tykerra | Colonial | 28.23 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | KRUEL, Peyton | Bishop Moore | 27.76 |
| 2 | DUNN, Kaylee | Bishop Moore | 27.49 |
| 3 | SMITH, Victorious | Lake Nona | 27.40 |
| 4 | BERNARD, Patley | Winter Park | 27.26 |
| 5 | DIAS, Skye | Eustis | 27.14 |
| 6 | FOSTER, Ava | Tavares | 27.16 |
| 7 | THOMPSON, Tailin | Timber Creek | 27.37 |
| 8 | SIMPSON, Madeline | Lake Highland Prep | 27.44 |
| 9 | TAYLOR, Danta'jia | Eustis | 27.70 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | PLATON, Olivia | Lake Nona | 27.14 |
| 2 | PIOLI, Isabella | Timber Creek | 26.48 |
| 3 | ROBERTS, Asia | South Lake | 25.94 |
| 4 | MORRIS, Emma | Lake Nona | 25.27 |
| 5 | LABORD, Morgan | Oak Ridge | 24.00 |
| 6 | MORILLO, Mariana | Horizon | 25.03 |
| 7 | PRUDENT, Castina | South Lake | 25.87 |
| 8 | IVA, Kylie | Lake Mary | 26.32 |
| 9 | JILES, Sofia | Lake Mary | 26.87 |