| Lane | Team | Squad | Seed |
|---|---|---|---|
| 4 | South Lake | A | 45.87 |
| 5 | Horizon | A | 44.62 |
| 6 | Boone | A | 45.87 |
| 7 | Eustis | A | NT |
| Lane | Team | Squad | Seed |
|---|---|---|---|
| 1 | Freedom (Orlando) | A | DNF |
| 2 | Oviedo | A | 43.56 |
| 3 | First Academy (Orlando) | A | 43.10 |
| 4 | Colonial | A | 43.00 |
| 5 | Lake Mary | A | 42.29 |
| 6 | Timber Creek | A | 42.80 |
| 7 | Bishop Moore | A | 43.01 |
| 8 | Winter Park | A | 43.10 |
| 9 | Cypress Creek | A | DNS |