| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | DAWKINS, Mikayla | Tohopekaliga | NT |
| 2 | GOMEZ, Vanelyn | Cypress Creek | 21.94 |
| 3 | TAYLOR, Ava | Eustis | 20.23 |
| 4 | SANCHEZ, Maria | Horizon | 19.90 |
| 5 | SNYDER, Brooke | Eustis | 19.53 |
| 6 | CLEMONS, Caitlin | Winter Park | 19.88 |
| 7 | ZAGROBELNY, Avana | Lake Mary | 19.93 |
| 8 | FRANCOIS, Eva | Tohopekaliga | 21.18 |
| 9 | WARD, Bethany | Eustis | 23.85 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | BARTIMUS, Emily | Winter Park | 18.96 |
| 2 | GINN, Azia | Horizon | 18.50 |
| 3 | LOULAN-WYCHE, Breanna | Lake Mary | 17.74 |
| 4 | OVERBY, Nia | Boone | 16.54 |
| 5 | MORILLO, Mariana | Horizon | 14.10 |
| 6 | REESE, Karis | Lake Mary | 14.56 |
| 7 | LANE, Claudia | Winter Park | 16.69 |
| 8 | MARRERO, Jayani | Lake Nona | 18.48 |
| 9 | RODRIGUEZ, Giselly | Colonial | 18.94 |