| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | McKeel |
A | 4:37.19 | Lakeland City Championships @ George Jenkins 2025 |
| 4 | Hardee |
A | 4:23.47 | Tenoroc Last Chance Meet |
| 5 | Manatee |
A | 4:26.03 | Hurricane Invitational Track and Field Meet |
| 6 | Ridge Community |
A | 4:46.56 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Bloomingdale |
A | 4:08.88 | FHSAA 4A Region 3 |
| 2 | Booker |
A | 4:06.14 | FHSAA 2A Region 3 |
| 3 | George Jenkins |
A | 4:00.90 | FSU Relays (High Schools) |
| 4 | West Port |
A | 3:51.00 | |
| 5 | Hillsborough |
A | 3:57.06 | |
| 6 | Zephyrhills |
A | 4:03.76 | FHSAA State Track & Field Championships (3A) |
| 7 | Dr. Phillips |
A | 4:06.47 | FHSAA 4A District 9 |
| 8 | Sebring |
A | 4:15.00 |