| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Frostproof |
A | DNS | FHSAA 2A Region 3 |
| 4 | Santa Fe Catholic |
A | 46.10 | 46.10 2025 Titan Invitational |
| 5 | Victory Christian (LL) |
A | 46.10 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Hardee |
A | 44.47 | FHSAA 2A Region 3 |
| 2 | Ridge Community |
A | 44.10 | |
| 3 | Auburndale |
A | DQ | |
| 4 | Haines City |
A | DQ | 43.98 Kowboy Invitational |
| 5 | Lakeland Christian |
A | 44.00 | |
| 6 | Lake Wales |
A | 44.01 | 44.01 2025 Titan Invitational |
| 7 | McKeel |
A | 44.33 | Lakeland City Championships @ George Jenkins 2025 |
| 8 | Kathleen |
A | 44.80 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Avon Park |
A | 43.78 | FHSAA 2A District 9 |
| 2 | George Jenkins |
A | DNS | FHSAA 4A District 6 |
| 3 | Sumner |
A | 42.86 | FHSAA 4A District 11 |
| 4 | Sebring |
A | 42.15 | FHSAA 3A Region 2 |
| 5 | Bloomingdale |
A | 42.81 | 42.81 Western Conference Federal Division 2025 |
| 6 | Dr. Phillips |
A | DNS | 42.93 Metro West Championships @ West Orange 2025 |
| 7 | Lakewood Ranch |
A | DNS | FHSAA 4A District 11 |
| 8 | Zephyrhills |
A | DNS | FHSAA 3A Region 2 |